FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376012
Sequences flagged as poor quality0
Sequence length2-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA124810.9070415083589387No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC49080.35668293590462874No Hit
CACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAAA35770.2599541283070206No Hit
TGTGGAGGAGAATGAATGGCTTCTACGGTGACGAATCAGTCATCCTTATT28730.2087917837925832No Hit
TCACAGTTTACATTCCCCAAAGAGAATTAACTAATTAAAAAGTTTAAAAA17080.12412682447536796No Hit
GTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACA17010.12361810798161643No Hit
TGTGGAGGAGAATGAATGGCATTTATGGTTTTAATTTTGTTAGTAAAGAC16710.1214378944369671No Hit
TGTGGAGGAGAATGAATGGCTTTTACGGTGATGAATTAGTTATTTTTATT15970.11606003436016546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGT706.5052336E-7183.96414138
GGCAGGC6200.0176.54622138
ACGCCGG100.008685334135.489728
AGTAGAC100.008685334135.489723
GGTAGGT2250.0114.466576138
GATTAGT8000.0100.605385138
GTTTACA47000.091.5276266
GGAGAAT42050.084.580387
AAGACAG32600.083.94069138
AGTTTAC54500.078.559175
GAGAATG45500.078.316038
TCACAGT54650.078.219591
ACAGTTT55900.076.591683
CACAGTT56850.074.835132
GACAGGG3153.1141099E-971.5416138
TTTACAT62600.069.584577
CTCCGTG150.00241789768.549416108-109
CGTATTC209.07601E-568.1186296-97
ACATCGC150.00252393267.81138672-73
GCTAGTA650.067.7448638-39
Produced by FastQC (version 0.11.5)