FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621812
Sequences flagged as poor quality0
Sequence length2-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA19730.3172984760667211No Hit
TGATAAAGGTCAGTTGAATGAATGATTAGAATTCCAATCTGCAAAAGACC7640.12286671855802075No Hit
ACAATAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA7470.12013277324979255No Hit
TGACAAAGGTCAGTTGAATGAATGATTAGAATTCCAATCTGCAAAAGACC6500.10453320296166686No Hit
TGACAAAGGTTAGTTGAATGAATGATTAGAATTCCAATCTGCAAAAGACC6430.10340746077592583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA152.6667627E-4433.63293138
GCCGCTT607.774409E-5162.61235138
GTCGCTT1451.0859367E-9134.57574138
TCTCGCA100.008892273134.420649
TTAACAA100.008892273134.420644
GTCGCCT751.8910738E-4130.08989138
TGATAAA8200.0112.290411
CAATAAC4950.0109.980522
AAGCTTT3300.0108.40824138
AAGGTTA9350.0107.8240366
ACAGTAA12200.0106.3245241
GCCGTCT2453.6379788E-12106.19582138
TAAAGGT9000.0102.3090444
CAGTAAC13300.097.530762
GTCAGTT8200.094.258389
AAAGGTT10750.093.781845
CAAAGGT9300.092.5045244
AGGCCGT1150.091.21298136-137
AAGGTCA8550.090.399836
AGTAACA14750.087.942993
Produced by FastQC (version 0.11.5)