FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621812
Sequences flagged as poor quality0
Sequence length2-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA25570.4112175384199726No Hit
ACAATAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA10360.16660984348967212No Hit
ACAGTAACAAACCCTGCTTCTTTATATTCAAGAGATATTCTGAACTCATA7560.121580156060031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCT759.094947E-12265.5382138
AAGCTTT3300.0231.3401138
AACTTTG856.2409526E-9195.24867138
TGGCGGG450.0067277267147.52122138
CCGCTTA452.6113456E-4147.52122138
AGGTCGT1450.0145.20844136-137
GCCGTCT3150.0136.98401138
TTATCAG100.008893735134.413242
CAATCCT100.008893735134.413243
GCCGCCT500.009218305132.7691138
TGATAAA10650.0113.5886461
ACAGTAA16750.0113.147861
CAATAAC5400.0110.7664642
GCCGCTT1604.7512003E-9103.72586138
CAGTAAC18550.0102.893152
AGTAACA19600.098.06683
AAGGTTA13750.096.7775346
GCCGTTT2757.2759576E-1296.55935138
ACAAACC26550.095.937137
AACAAAC27350.094.113846
Produced by FastQC (version 0.11.5)