FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702091
Sequences flagged as poor quality0
Sequence length20-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAGGCAGACACTAA413221.5292601174423808No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT320291.1853412782915158No Hit
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG165910.6140059679707308No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTCTTTCC113980.4218214708534983No Hit
ACCCATCCACACCTTCCTATTTTCCACAGGACAACCAGACAAACTTTTCA90630.33540691264653927No Hit
ACCCATCCACACCTTCCTCATGGTGCCCACTCTACTCTGGCCACACTGGT67530.2499175638422244No Hit
GGGTAGAAAGCAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT64350.23814890024059146No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT62890.23274567732914989No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGTAGTTTTTTTTTTT49070.18160010155098405No Hit
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAAACAAGCACTAA44470.1645762485423326No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCGTGATTTCCACTTGGCCAGACCGTC34710.1284560734631069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACT850.0284.20993138
TGGGACC12400.0265.44406138
CACATTT6550.0175.19048138
TAGGACC6950.0160.76263138
TCCGCAC100.008650765135.673026
ATCCGCA100.008650765135.673025
CCATCCG100.008650765135.673023
CATCCGC100.008650765135.673024
CCGCACC100.008650765135.673027
ATCCACA174250.0128.315145
CATCCAC175100.0127.653524
TCCACAC181650.0123.1625756
CCACACC181950.0122.996797
TGGAACC7100.0119.08797138
GGGTAGA124200.0118.249641
GTAGAAA125750.0117.924953
AGTAAAG118200.0108.81399
ACCCATC211100.0106.269711
CACACCT224650.099.527778
ACACCTT226150.098.897629
Produced by FastQC (version 0.11.5)