FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702091
Sequences flagged as poor quality0
Sequence length20-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAGGCAGACACTAA357341.3224573117633713No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT350561.2973656327636633No Hit
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG152150.5630824424491995No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTCTTTCC122590.4536856826805611No Hit
GGGTAGAAAGCAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT73200.27090131309419263No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT69720.2580223982093867No Hit
ACCCATCCACACCTTCCTCATGGTGCCCACTCTACTCTGGCCACACTGGT60600.22427075920092993No Hit
ACCCATCCACACCTTCCTATTTTCCACAGGACAACCAGACAAACTTTTCA59350.21964471218770945No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGTAGTTTTTTTTTTT55090.20387914396665396No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCGTGATTTCCACTTGGCCAGACCGTC36690.13578373193204818No Hit
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAAACAAGCACTAA34180.1264946295295014No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTCTTTCT31800.11768663601632959No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTCTTTCT29550.10935975139253268No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGTAGTTTTTTTTTCT28760.10643608968017731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC13800.0270.26715138
ACTAACT1100.0250.12804138
CACATTT7650.0179.8306138
CGGCATT151.6045614E-4135.644471
ATCCACA144500.0128.134755
CATCCAC143750.0128.048394
TCCACAC150350.0123.329566
CCACACC151000.0122.484267
GTAGAAA139250.0118.9872363
TGGAACC7200.0118.888016138
GGGTAGA140650.0116.50091
AGTAAAG127400.0113.338749
TGAGACC9550.0108.83989138
GGCATTC259.008749E-6108.515572
ACCCATC178000.0103.333661
ACACCTT189500.098.0648659
CACACCT190550.097.3465358
GGTAGAA174900.094.462532
AGCAAAG23700.092.719019
AGCACAT25450.089.981186136-137
Produced by FastQC (version 0.11.5)