FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610166
Sequences flagged as poor quality0
Sequence length2-144
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCACC36800.083.362759
GCCTCTT37200.070.436393
CAGCCTC38100.069.453461
AGCCTCT41750.063.3814772
ACTTAGG53200.051.2031143
CTTAGGA53750.051.1618274
GGTGAAT46200.050.58077128-129
TCACCAC34200.049.59115610-11
TACGCGA401.1285101E-649.47097878-79
TCGTCCG200.00929748648.7801258-59
GAACTTA59300.047.9046971
ACGATTG25050.047.6740946-47
TAACGAT25400.047.6504644-45
TACACGA12800.046.63670378-79
TTAACGA25650.042.75838544-45
CACACGC19800.041.27280824-25
AAGGGAG50950.041.24387714-15
TTTAACG29600.040.99210442-43
CCTCTTC76650.040.6997384
TGGTGAA53700.040.309944128-129
Produced by FastQC (version 0.11.5)