FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169387
Sequences flagged as poor quality0
Sequence length8-144
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGTCAGCCTCTCTAAGGAAAAGAGGACACGGCCTCAGGGCACTCTGG322851.4882084201666186No Hit
ACTCGGACTCTGCCAGAAAAACACCAATAAGAAAACTAAAAACAACCACC321381.4814323124458662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGA34000.0132.830251
CTCGGAC34750.0132.280732
ACCGTCA34250.0132.252532
CGGACTC34950.0132.099784
TCGGACT35000.0131.335883
CCGTCAG34450.0130.900383
CGTCAGC34650.0130.144824
GGACTCT35650.0129.694185
TACCGTC259.496403E-6107.369471
CACCGTC45200.0100.065021
GTCAGCC46700.096.850945
CCGTAAG354.379017E-795.865593
GCCTCTC47750.095.434919
TGCAAAA56700.091.979675138
CGTAAGC409.695232E-783.882394
ATACGTC2850.080.0654149
ATTGCAA42050.076.0383136-137
ACCGTAA451.9530034E-674.562132
AACGATT35450.067.53527120-121
ACGATTG35350.067.35193122-123
Produced by FastQC (version 0.11.5)