FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032440
Sequences flagged as poor quality0
Sequence length4-144
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA168711.6340901166169461No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC107501.0412227344930456No Hit
CACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAAA24180.23420247181434273No Hit
GTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACA17080.1654333423734067No Hit
GGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACAGT12460.12068497927240324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGC7550.0137.30974138
ACAGTTT24450.0112.9714053
GTTTACA25200.0108.801226
TTTACAT26200.0105.684637
GAGAATG19400.090.604768
GGAGAAT19800.088.774357
GGCGAAT250.00122023281.439347
ACGGCAG350.001303416780.09561136-137
CACAGTT35400.077.643442
AGTTTAC37300.074.05235
TCACAGT39400.069.760851
TTACATT40850.068.945998
GCGAATT150.002515783867.8661122-23
AACGGTT150.002515783867.8661138-39
AAGACAG28300.066.11648138
GGAAGAC23450.064.86034136-137
TACATTC47600.059.0264139
GACTAAT26400.058.48310528-29
TTGACTA26650.058.44380626-27
CTAATTG27050.058.0813530-31
Produced by FastQC (version 0.11.5)