FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979307
Sequences flagged as poor quality0
Sequence length5-144
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA172721.7636961647369005No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC114581.1700110384179834No Hit
CACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAAA23180.23669799153891477No Hit
GTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACA16720.17073297750347952No Hit
GGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACAGT11140.1137539096524379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG50.00453379331343.5267138
GGCAGGC7500.0206.00742138
ACAGTTT23550.0111.3776253
GTTTACA23500.0111.326946
TTTACAT23950.0109.517467
GAGAATG20800.098.8023768
GGAGAAT21200.096.938187
AAGACAG27250.093.677086138
GGAAGAC16800.077.98225136-137
AGTTTAC35600.074.247855
AGACAGG18450.069.17888138
TTACATT38750.068.910058
CACAGTT38600.068.127032
AGAATGA33550.061.657529
TACATTC44500.060.309779
TCACAGT43500.060.2975431
GGCCTTA18200.059.61572326-27
CTATGGC18100.059.571622-23
ATCTATG18200.059.43000420-21
GTTCCCG17500.058.94008666-67
Produced by FastQC (version 0.11.5)