FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22861886
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAC43650.019.91864214-15
CCTAAGG112150.019.3129238
GGAACGA53450.016.86036112-13
ACGACAT52050.016.44784416-17
TCCTAAG134600.016.2462657
AACGACA54750.016.13855714-15
CGACATG55850.016.0043916-17
CTAAGGA140100.014.3574779
AGGAACG69050.013.97127312-13
CGATCCG16500.013.51262528-29
CTTCCTA184550.012.6476035
GATCCGA31800.012.54647928-29
TACGATC19150.012.39219726-27
TTCCTAA195350.011.9463796
ATCCGAC27000.011.38695628-29
GGCTACG32350.010.92101722-23
GTCTTCC215550.010.9080773
TCCGACT42200.010.35351430-31
ACGATCC30450.09.95401126-27
CTACGAT27900.09.3543424-25
Produced by FastQC (version 0.11.5)