FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23329654
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG144400.034.268828
TCCTAAG170400.029.5355347
GAACGAC65350.028.79052414-15
CTAAGGA166700.027.118839
ACGACAT73100.026.41682816-17
GGAACGA79650.024.60485812-13
CGACATG80700.024.42387616-17
AACGACA76950.024.2978114-15
CTTCCTA225950.022.8389285
AGGAACG96650.021.9526412-13
TTCCTAA236150.021.4816176
TACGATC32200.021.3495226-27
GTCTTCC246950.020.9486163
GGCTACG47900.020.7947522-23
CTACGAT36950.020.40169724-25
GATCCGA48250.020.1804328-29
ACGATCC46500.019.72877726-27
CGATCCG33250.018.98192428-29
GCTACGA39350.017.9637724-25
TCCGACT63100.017.62208430-31
Produced by FastQC (version 0.11.5)