FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20634976
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG94250.017.3729348
GAACGAC37150.015.80700614-15
TCCTAAG116500.014.3124677
ACGACAT41650.013.65962916-17
CGACATG46050.013.020916-17
GGAACGA47500.012.83011312-13
CTAAGGA122000.012.6092479
AACGACA46950.012.55779714-15
AGGAACG62050.011.26819412-13
CTTCCTA164600.010.8451395
GTCTTCC173650.010.5025473
GGCTACG26200.09.80338122-23
TTCCTAA176900.09.7946166
GATCCGA25700.09.71693928-29
TACGATC16600.09.50596326-27
ATCCGAC23700.09.4434828-29
CGATCCG14600.09.35905328-29
TGTCTTC245000.07.73488332
TCCGACT35100.07.712587430-31
TTGTCTT242600.07.52133851
Produced by FastQC (version 0.11.5)