FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177365292
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG71750.018.44401226-27
GAACGAC154800.018.37945712-13
ACGACAT217300.013.66035814-15
TACGATC126250.012.26111924-25
GCTACGA131450.012.19979422-23
ATCCGAC104500.011.76524428-29
CGACATG216650.011.02726316-17
AGGAACG319050.010.512410-11
GGAACGA245850.09.64867512-13
GGCTACG154400.09.53357522-23
AACGACA245400.09.25225914-15
CTACGAT146750.07.983381324-25
CCTAAGG791050.07.7440037
CCGACTT178600.07.00259430-31
GATCCGA123000.06.83513828-29
TCCGACT156600.06.37053328-29
GTCTTCC1198900.06.12111332
TCCTAAG1111000.05.9990196
CTAAGGA1000350.05.91379458
ACGATCC205500.05.54751926-27
Produced by FastQC (version 0.11.5)