FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116648689
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGATCGTAGCCATGTCGTTCCTTAGGAAGACAAAAGTCGGATCGTA2249130.19281228269955097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG1488250.019.0474417
GATCGTA1490300.019.0421186
CGTAGCC1507550.018.928669
TCGTAGC1503100.018.9106628
CGGATCG1517950.018.777454
TCGGATC1554800.018.328663
GGATCGT1564500.018.2095725
GTCGGAT1572200.018.1803042
CGCGACG109650.017.93148244-45
AGTCGGA1626350.017.7947521
CGACGTT109150.017.72347546-47
CGTTCCT980850.017.71920-21
CGATCGT19250.016.8315354
TCGATCG19200.016.6411023
GTCGTTC1292950.015.9160394
AACGCGA116250.014.97966742-43
TCGTTCC951850.014.85678218-19
AAAGTCG959450.014.23498336-37
GTCGGTC72950.014.0949694
CCTTAGG1268300.013.94039124-25
Produced by FastQC (version 0.11.5)