FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117393776
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGATCGTAGCCATGTCGTTCCTTAGGAAGACAAAAGTCGGATCGTA2911910.2480463700222063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG18800.020.9529823
GATCGTA1763650.020.305276
CGGATCG1779700.020.2304534
ATCGTAG1774600.020.1223727
TCGTAGC1789650.020.0107238
CGTAGCC1790050.019.984749
GTCGGAT1807250.019.8415552
TCGGATC1801900.019.8259833
GGATCGT1819350.019.7873785
CGCGACG91700.019.6759418-19
AGTCGGA1850600.019.5986671
CGATCGT21350.018.8888234
CGACGTT92000.018.71629720-21
CGTTCCT1234150.018.6642720-21
GCGACGT93300.017.87720520-21
GTCGTTC1592450.017.76886794
ACGCGAC90250.017.61702518-19
GTCGGTC70300.016.81006894
AACGCGA99300.016.7933916-17
TCGTTCC1217650.015.81978818-19
Produced by FastQC (version 0.11.5)