FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122470516
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGATCGTAGCCATGTCGTTCCTTAGGAAGACAAAAGTCGGATCGTA1460990.11929320196544285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCC1039850.017.1560129
TCGTAGC1040700.017.1379668
GATCGTA1043200.017.0136876
ATCGTAG1043850.016.98887
CGGATCG1055600.016.887114
CGTTCCT687600.016.43096720-21
GTCGTTC875350.016.36016594
TCGGATC1101200.016.0864263
GGATCGT1109850.016.0651535
GTCGGAT1113900.015.9973732
AGTCGGA1167450.015.4749361
TCGTTCC649200.014.68105518-19
CGCGACG67950.013.86771918-19
CGACGTT69350.013.51965220-21
AAAGTCG688450.013.41573736-37
GTCGGTC64600.012.8937994
ACGCGAC67650.012.75112418-19
GCGACGT72050.012.45996420-21
ATGTCGT980950.012.26763616-17
CCTTAGG995250.011.73427424-25
Produced by FastQC (version 0.11.5)