FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54636281
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11422550.077.449881
GTGTTGG11738850.075.134782
TGTTGGG11945500.072.5447543
GTTGGGT12444300.069.456754
TGGGTGT13525650.063.9661946
TTGGGTG13800550.063.2746775
GGGTGTG14059150.061.2692877
GGTGTGT15076550.056.8223958
GTGTGTT15832250.053.8729449
GTTGCGT52750.032.2985084
TGTTGCG63600.027.000773
TGTGTTT15850950.026.68610810-11
GTGTTTG18069000.025.10045610-11
TTGCGTG77750.021.876395
GCGTTGG40700.021.557312
GTTTGGT12149200.021.4196612-13
TGTTTGG20625800.020.32295212-13
GTTTGGG8593600.020.03262512-13
CGTTGGG42500.019.7589113
GCGTGTT54800.019.6504049
Produced by FastQC (version 0.11.5)