FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33024130
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG2545900.049.5492131
GTGTTGG2612450.048.2461852
TGTTGGG2702700.046.2678383
GTTGGGT2728550.045.73374
TGGGTGT3085950.040.4899026
TTGGGTG3128200.040.108665
GGGTGTG3295400.037.8628047
GGTGTGT3758500.033.195648
GTGTGTT4052700.030.8107859
GTGTTTG3911950.017.7060710-11
GTTTGGT2412400.016.18892512-13
TGTTTGG3853700.015.97984312-13
GTTTGGG1908600.015.62734112-13
TGTGTTT4115300.015.16084110-11
TTGGGGG907350.013.552875514-15
GCCTTCG51000.011.83719774-75
TTGGTTT1795350.011.64279914-15
TTTGGGG1390550.011.39873314-15
TTTGGTT2180850.011.13351814-15
CTATTAA282350.010.888461
Produced by FastQC (version 0.11.5)