FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44940047
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG10336600.066.160611
GTGTTGG10746050.063.575922
TGTTGGG10933300.061.8587843
GTTGGGT11462550.058.7848244
TGGGTGT12484000.054.0782136
TTGGGTG12854550.053.1595845
GGGTGTG13331400.050.5190357
GGTGTGT13967300.048.0430378
GTGTGTT14486800.046.238189
TTGGTTT5841650.023.7055993
TGTGTTT14089850.023.6471310-11
TGGTTTT5304950.023.3460164
GTGTTTG16056050.022.01357310-11
TTTGGTT7129750.021.587012
GTTTGGA1580150.021.26587112-13
TGTTTGG15996200.020.6370412-13
GGTTTTT5355250.020.5366785
GTTTGGG7349700.018.86038812-13
GTTTGGC999400.018.40291412-13
GAACGAC54800.018.11394594
Produced by FastQC (version 0.11.5)