FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55593109
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG8794400.064.299491
GTGTTGG9347200.059.9269942
TGTTGGG9499550.058.3711173
GTTGGGT9980800.055.4654354
TGGGTGT11307350.049.6373636
TTGGGTG11602600.048.9054075
GGGTGTG12378950.045.4188237
GGTGTGT13219500.042.227588
GTGTGTT13951350.039.8530659
GTTGCGT63050.028.901484
TTGGTTT6407500.027.8462943
TGGTTTT5821250.027.4346854
TTTGGTT7699050.025.6582812
GGTTTTT5963100.023.8481225
TGTTGCG82650.023.048943
TTGCGTG86550.021.5552025
GTTTGGT10497450.021.1617771
CATAACG162900.020.5876733
TGTGTTT13740400.020.19659610-11
GTGTTTG15954200.018.8863110-11
Produced by FastQC (version 0.11.5)