FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55709631
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12153600.063.6805231
GTGTTGG12882900.059.62032
TGTTGGG13314250.056.3163873
GTTGGGT14091600.052.8261534
TTGGGTG15880500.047.526025
TGGGTGT15733050.047.461936
GGGTGTG17052950.043.5995987
GTTGCGT77900.041.784224
GGTGTGT18935400.039.1527678
TGTTGCG89050.037.1048553
GTGTGTT20044250.036.967999
TTGCGTG100700.033.0803075
TGCGTGT141550.024.331786
GCGTGTG154150.022.1234047
CGTGTGT158800.021.3868038
GTGTTTG21763800.019.16311810-11
TGTGTTT19342300.019.07471810-11
GTTTGGT13537300.016.71167412-13
TGTTTGG22125600.016.59343312-13
ACGACAT113550.015.38783894
Produced by FastQC (version 0.11.5)