FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55031975
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11411800.071.528191
GTGTTGG11805950.067.8672942
TGTTGGG12036000.065.021233
GTTGGGT12719800.061.206154
GTTGCGT145400.057.1287574
TGGGTGT14119700.055.4703456
TTGGGTG14314600.055.1632965
TGTTGCG154700.054.094153
GGGTGTG14883750.052.4554677
TTGCGTG168050.050.085595
GGTGTGT16712050.046.6132478
GTGTGTT17966000.043.4842649
GCGTGTG215450.041.2988247
TGCGTGT218300.039.8727656
CGTGTGT237950.036.8104328
GTGTCGG50200.022.4488092
TGTGTTT17689250.021.96206910-11
GTGTTTG19965400.021.55037510-11
GTGTTGC412700.020.8437922
TGTCGGG52950.020.1453293
Produced by FastQC (version 0.11.5)