FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71592954
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14540600.072.202621
GTGTTGG15302250.068.234332
TGTTGGG15682300.065.056763
GTTGGGT16585500.061.186844
TGGGTGT18489200.055.107776
TTGGGTG18610800.055.07475
GGGTGTG19862750.051.1072777
GGTGTGT22422400.045.038068
GTGTGTT23882900.041.834599
GTTGCGT88700.037.962964
TGTTGCG100500.033.823293
TTGCGTG113250.030.1898175
TGCGTGT161000.022.1223166
GTGTTTG25561300.021.46509410-11
TGTGTTT23244950.021.2647510-11
GCGTGTG177600.020.2157657
GTTTGGT15918300.019.5452412-13
TGTTTGG25865600.018.94407512-13
CGTGTGT195200.018.1924768
TTGGTTT8533300.017.404214-15
Produced by FastQC (version 0.11.5)