FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62975392
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG18776700.081.25941
GTGTTGG19829600.076.686632
TGTTGGG20898900.071.512223
GTTGGGT22452500.066.253974
TTGGGTG25042350.059.956135
TGGGTGT24926550.059.737376
GGGTGTG26687100.055.6591457
GGTGTGT28813600.051.379948
GTGTGTT29787150.049.5602659
GTTGCGT58900.034.0238884
TGTTGCG70200.028.3969963
TTGCGTG78850.025.625155
TGTGTTT29238750.025.02672610-11
GTGTTTG32708800.024.44116610-11
GTTTGGT20492300.022.26525512-13
TGTTTGG32887900.022.08525312-13
TTGGTTT12874650.020.16435614-15
GTGTCGG41400.019.7985252
GTTTGGC1235650.019.69740312-13
GTTTGGG14177600.019.51202612-13
Produced by FastQC (version 0.11.5)