FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52254767
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG10778450.071.21841
GTGTTGG11081800.067.960212
TGTTGGG11370700.064.9674153
GTTGCGT130650.062.239974
GTTGGGT12140750.060.693254
TTGGGTG12878450.057.8514565
TGGGTGT12892750.057.5843666
TGTTGCG143700.057.0148243
TTGCGTG147250.055.911545
GGGTGTG13510550.054.8162967
GGTGTGT14717450.049.5437668
GTGTGTT15423950.046.0351339
CGTAGCC32500.044.74820794
GCGTGTG193000.044.327837
CGTGTGT202000.041.2969978
CGATCCG65100.034.88759674-75
ATCCGAC65300.033.02893476-77
TGCGTGT277000.031.7643326
CGACTTA63550.031.23310578-79
GATCCGA66600.030.59968474-75
Produced by FastQC (version 0.11.5)