FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58498430
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG15841200.077.861061
GTGTTGG16276000.074.80062
TGTTGGG16629500.071.935083
GTTGGGT17443750.068.3024754
TGGGTGT18868600.063.646076
TTGGGTG19040400.063.1252335
GGGTGTG19868000.060.7457357
GGTGTGT21043000.056.744258
GTGTGTT21525900.053.9698379
GTTGCGT117450.051.701364
TGTTGCG118800.050.7223553
TGTCGGG86400.048.237713
GTGTCGG88650.048.1575162
TTGCGTG132800.045.959645
TGTGTCG117100.040.9577941
TCGGGTG112400.037.5995065
GCGTGTG185950.037.0025257
CGGGTGT124650.033.288946
CGTGTGT200350.032.607848
GTGTGTC563000.031.6328499
Produced by FastQC (version 0.11.5)