FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63448727
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12876550.073.241421
GTGTTGG13238850.069.7976462
TGTTGGG13650350.066.559313
GTTGCGT174950.064.4126364
GTTGGGT14530750.062.346284
TGTTGCG192450.058.5286183
TTGGGTG15626200.058.5181545
TGGGTGT15648050.058.334526
TTGCGTG201750.056.3086175
GGGTGTG16542900.055.0571447
GGTGTGT18060100.049.6526578
GTGTGTT19015050.046.0627569
GCGTGTG262750.043.778397
CGTGTGT265550.042.355388
TGCGTGT279650.041.6638536
GTGTGTC539500.027.838119
GTGTTGC451300.025.5124382
TGTGTTT18697300.023.19146710-11
GTGTTTG21509400.021.9473510-11
TGTTTGG21711250.019.8422312-13
Produced by FastQC (version 0.11.5)