FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60618354
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG16561400.068.56421
GTGTTGG17251900.065.43592
TGTTGGG17766650.062.3852353
GTTGGGT18739350.058.904634
TTGGGTG19496150.057.3361175
TGGGTGT19366950.057.1828086
GGGTGTG20246650.054.473777
GGTGTGT21976700.049.780648
GTTGCGT77950.047.7570154
GTGTGTT23095600.046.7288749
TGTTGCG88200.042.4204373
TTGCGTG91850.040.9311875
GCGTGTG129150.030.6456787
CGTGTGT152900.025.4356468
TGTGTTT21736550.024.52476310-11
CGTAGCC18050.023.05856394
CGACATG297500.022.54322294
GTGTTTG25973250.022.2571210-11
CGATCCG31600.021.26323174-75
ACGACAT384250.021.1833194
Produced by FastQC (version 0.11.5)