FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51660544
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11778000.078.198911
GTGTTGG12164400.075.090092
TGTTGGG12447350.072.081143
GTTGGGT12992850.068.744984
TGGGTGT14114400.063.4366466
TTGGGTG14299300.063.11685
GGGTGTG14762000.060.5738837
GGTGTGT15934300.055.805458
GTGTGTT16722850.052.8831189
GTTGCGT61500.044.2424554
TGTTGCG67000.040.6813473
TTGCGTG73450.037.297565
GTGTCGG46600.036.772032
TGTCGGG48550.034.0337223
GCGTGTG107450.029.3988257
TGTGTCG67150.029.3975621
TGTGTTT16593750.026.34311510-11
CGGGTGT61500.026.0217536
TCGGGTG65100.025.3067465
GTGTTTG19096100.024.55721310-11
Produced by FastQC (version 0.11.5)