FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89363284
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG26952150.068.3598251
GTGTTGG27946100.065.6137852
TGTTGGG28740450.062.8011253
GTTGGGT30381200.059.1754074
TTGGGTG30781500.058.952725
TGGGTGT30682200.058.713916
GGGTGTG31964600.056.1219067
GGTGTGT34662150.051.3222928
GTTGCGT103500.049.3940964
GTGTGTT36440400.048.128939
TGTTGCG115550.044.3010183
TTGCGTG124400.041.843055
GCGTGTG177850.030.4420287
CGTGTGT209500.025.23958
TGTGTTT34390450.025.21664610-11
GTGTTTG40477400.023.05049310-11
CGACATG341850.022.66521894
ACGACAT451650.021.97278694
TGTTTGG41188750.020.99986312-13
GAACGAC716900.020.99535494
Produced by FastQC (version 0.11.5)