FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60498557
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG10963950.074.122311
GTGTTGG11285200.070.7494352
TGTTGGG11590800.067.7131963
GTTGGGT12403450.063.062974
GTTGCGT129550.060.7763984
TGGGTGT13569200.058.7586026
TTGGGTG13563050.058.294255
TGTTGCG141000.056.209233
GGGTGTG14381550.055.4365737
TTGCGTG150250.053.1785935
GGTGTGT15666450.050.2898568
GTGTGTT16543800.046.332839
GCGTGTG206850.040.6906177
CGTGTGT221850.037.185868
TGCGTGT234600.035.8523336
GTGTCGG60100.029.075412
GTGTGTC506400.028.4515539
CGTAGCC28450.027.09747194
GTCGGGT60750.026.8950444
CGATCCG44450.026.41571274-75
Produced by FastQC (version 0.11.5)