FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50440132
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12439500.068.140791
GTGTTGG12906450.064.7587662
TGTTGGG13221250.061.939853
GTTGGGT13994850.058.258694
GTTGCGT114150.058.2564544
TGGGTGT15232950.054.479556
TGTTGCG123200.054.3158763
TTGGGTG15191400.054.293375
TTGCGTG127050.052.7866485
GGGTGTG16168750.051.3382647
GGTGTGT17727150.046.2614868
GTGTGTT18651000.042.6835869
GCGTGTG182150.038.2350967
CGTGTGT202300.033.781118
GTGTCGG59000.032.1720162
TGCGTGT218050.032.105456
GTCGGGT57550.031.9887624
TGTCGGG60350.030.6015053
CGTAGCC17900.029.9368594
TCGGGTG68800.027.5394745
Produced by FastQC (version 0.11.5)