FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50440132
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14231600.076.279671
GTGTTGG14796300.072.802562
TGTTGGG15111500.069.8828053
GTTGGGT15750800.066.794674
TGGGTGT17063900.061.9320346
TTGGGTG17307700.061.524385
GGGTGTG18037500.058.5898447
GGTGTGT19357150.054.217158
GTGTGTT20138850.051.521939
GTTGCGT70650.046.5963484
TTGCGTG79000.042.29535
TGTTGCG80750.041.206123
TGTCGGG47900.035.517633
GTGTCGG51150.034.0262
GCGTGTG120850.032.3960767
TGTGTCG71700.028.042791
CGTGTGT134900.027.5574478
TCGGGTG65750.026.2706765
TGTGTTT19643600.026.05423410-11
GCGTTGG38500.025.1687322
Produced by FastQC (version 0.11.5)