FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53308504
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12938900.072.9058151
GTGTTGG13542750.069.298332
TGTTGGG13911250.065.8535843
GTTGGGT14673250.062.088024
TTGGGTG16683300.055.4855355
TGGGTGT16567350.055.3866466
GGGTGTG17838450.051.2311257
GGTGTGT20330400.044.8078888
GTGTGTT21358300.042.466399
GTTGCGT67600.022.4138344
TGTGTTT20730950.021.73265610-11
GTGTTTG23234000.021.2229210-11
TGTTTGG23315000.019.22373412-13
GTTTGGT15287650.019.17824712-13
TTGCGTG82050.019.0698815
TGTTGCG82500.018.0429273
TTGGTTT8368000.017.44664214-15
GTTTGGG10855250.016.65524512-13
TTTGGTT10993100.016.49087714-15
TGTTGGT1454250.016.0608443
Produced by FastQC (version 0.11.5)