FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79510344
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12360450.073.8639761
GTGTTGG12994150.069.908112
TGTTGGG13266450.067.305493
GTTGGGT13913200.063.8060844
TGGGTGT15477050.057.5744936
TTGGGTG15609750.057.464185
GGGTGTG16627100.053.4190187
GGTGTGT18058650.049.0336238
GTGTGTT18913950.046.7142339
GTTGCGT78300.032.113214
TGTTGCG90800.027.7326073
TTGCGTG102950.025.0340275
TGTGTTT18737100.023.55125810-11
GTGTTTG20394550.023.5044610-11
TGTTTGG20463200.021.42672312-13
GTTTGGT13125100.021.15494712-13
GTTTGGG10202950.018.84274312-13
TTGGTTT7418600.018.05960514-15
TTTGGTT9611600.017.5595614-15
TTTGGTG5313100.016.30525814-15
Produced by FastQC (version 0.11.5)