FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61860885
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12965300.071.671221
GTGTTGG13725950.067.12542
TGTTGGG14196300.063.703213
GTTGGGT15070350.059.7664644
TGGGTGT16647850.054.5537536
TTGGGTG16920400.054.0429845
GGGTGTG17884500.050.6021357
GTTGCGT108050.049.885054
TGTTGCG120350.045.077473
GGTGTGT20162450.044.6605648
TTGCGTG131250.042.031935
GTGTGTT21340200.041.4024629
TGCGTGT182850.030.7830856
GCGTGTG194100.028.8295787
CGTGTGT206800.026.5972968
GTGTGTC503500.021.1590069
TGTGTTT20803850.021.10917310-11
GTGTTTG23710800.020.39037110-11
GTGTCGG55900.018.3749962
TGTTTGG24002850.018.20153412-13
Produced by FastQC (version 0.11.5)