FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69845078
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG9831100.070.909991
GTGTTGG10278950.067.335172
TGTTGGG10605700.063.9845353
GTTGGGT11174450.060.4529724
TGGGTGT12422750.055.127656
TTGGGTG12616500.054.4509545
GGGTGTG13434300.050.6325877
GGTGTGT15332650.043.987028
GTGTGTT16533900.040.3711439
GTTGCGT69150.031.1360384
TGTTGCG79950.027.290313
TTGCGTG85700.026.0611675
TGTGTTT16466350.020.11461810-11
GTGTTTG19014450.019.09009710-11
TGCGTGT127250.018.2154036
TGTTTGG19248800.017.077312-13
GCGTGTG137550.016.9673187
TTGGTTT7003550.016.3573323
GAACGAC96900.016.14023894
GTTTGGT12737800.016.08788312-13
Produced by FastQC (version 0.11.5)