FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51763135
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12753100.082.335251
GTGTTGG13207350.078.910622
TGTTGGG13307500.076.9850853
GTTGGGT13967700.072.981234
TTGGGTG15817300.064.8855445
TGGGTGT15739000.064.701246
GGGTGTG16582800.061.237757
GGTGTGT17473150.057.959468
GTGTGTT17967800.056.414029
GTTGCGT89100.044.3431664
TTGCGTG99700.039.957335
TGTTGCG112500.035.3978733
TGTGTTT18071300.027.76266310-11
GCGTGTG156000.027.5492927
GTGTTTG20094700.026.71134610-11
TGCGTGT157150.026.630696
CGTGTGT158400.026.5228868
GTTTGGT12039850.024.77107212-13
TGTTTGG20039800.024.76342212-13
TTGGTTT6752950.021.81042914-15
Produced by FastQC (version 0.11.5)