FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52917632
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG10288350.080.25131
GTGTTGG10602000.077.4038542
TGTTGGG10749450.075.056243
GTTGGGT11346550.070.739074
TTGGGTG12957750.062.398355
TGGGTGT12954750.061.9215776
GGGTGTG13683300.058.4642837
CCTAAGG325350.057.03647
GGTGTGT14583350.054.621538
GTGTGTT15129850.052.3653039
CTAAGGA329700.048.1929558
CTTCCTA468350.039.405494
TCCTAAG470050.038.8107156
GTTGCGT59850.038.4091074
GGAACGA188450.037.85304612-13
GTCTTCC493950.037.4844362
TTCCTAA501150.036.130435
AGGAACG203550.035.98681610-11
TGTTGCG73550.032.1233633
TTGCGTG73950.031.16525
Produced by FastQC (version 0.11.5)