FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58842261
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG13980550.082.687781
GTGTTGG14302250.079.956662
TGTTGGG14441200.077.767153
GTTGGGT15112100.073.9408654
TTGGGTG16940700.066.348315
TGGGTGT16855650.066.1895456
GGGTGTG17543950.063.3994647
GGTGTGT18732150.059.203058
GTGTGTT19510950.056.973559
GTTGCGT99450.054.0362854
TGTTGCG111750.047.9206433
TTGCGTG124100.043.8333175
TGCGTGT180250.032.1299746
GCGTGTG193250.030.6684387
CGTGTGT205050.028.3446048
TGTGTTT19908200.027.67483510-11
GTGTTTG21864500.026.8444910-11
GTTTGGT13981450.025.00381312-13
TGTTTGG22423700.024.24060412-13
TTGGTTT8351150.021.89618914-15
Produced by FastQC (version 0.11.5)