FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50366240
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11153050.081.4079061
GTGTTGG11411350.078.628822
TGTTGGG11484400.076.303913
GTTGGGT12011450.072.525224
TTGGGTG13502900.064.97645
TGGGTGT13520400.064.360576
GGGTGTG14189800.061.1043177
GGTGTGT15359950.056.287588
GTGTGTT16156050.053.692179
GTTGCGT94900.049.542864
TGTTGCG104100.045.886843
TTGCGTG114050.041.8841865
GCGTGTG177350.029.3020327
TGCGTGT176200.028.34246
CGTGTGT184700.027.3346618
TGTGTTT16473600.026.13970410-11
GTGTTTG18095050.025.58607510-11
TGTTTGG18082750.023.46124312-13
GTTTGGT11645550.023.37572712-13
GTTCGGT43800.020.8037094
Produced by FastQC (version 0.11.5)