FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005751994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62756763
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG16648500.084.0910641
GTGTTGG17040350.081.271172
TGTTGGG17232250.078.923073
GTTGGGT18023400.075.060034
TTGGGTG20363750.066.7305455
TGGGTGT20319050.066.438116
GGGTGTG21268450.063.3134887
GGTGTGT22376000.060.0060428
GTGTGTT22896300.058.8007979
GTTGCGT124700.052.9930344
TGTTGCG141350.046.884053
TTGCGTG149600.044.832355
GCGTGTG214100.034.010287
TGCGTGT218650.032.3885656
CGTGTGT227100.031.2506628
TGTGTTT23261000.028.77086410-11
GTGTTTG25379650.028.03595210-11
TGTTTGG25255900.026.19884712-13
GTTTGGT15892900.026.17542612-13
TTGGTTT9334100.022.87283514-15
Produced by FastQC (version 0.11.5)