FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6057775
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG874100.077.070431
GTGTTGG916250.072.798442
TGTTGGG932550.069.720043
GTTGGGT985800.065.4769064
TTGGGTG1144750.057.2594645
TGGGTGT1152950.056.192936
GGGTGTG1258200.051.2731177
GGTGTGT1414800.045.418918
GTTGCGT9400.045.106524
GTGTGTT1519400.041.911769
TGTTGCG10300.041.507753
TTGCGTG12800.033.581015
TGCGTGT16850.024.6400246
GCGTGTG18600.022.828297
CGTGTGT19900.020.5960358
TGTGTTT1545050.020.4276110-11
GTGTTTG1763900.019.83162310-11
GTTTGGT1099400.017.90932712-13
TGTTTGG1771300.017.64375112-13
TTGGTTT628100.015.72823914-15
Produced by FastQC (version 0.11.5)