FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005751998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005751998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57816429
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG8913150.061.749611
GTGTTGG9262450.058.957392
TGTTGGG9511700.056.7260323
GTTGGGT10073100.053.456284
TGGGTGT11245050.048.6505176
TTGGGTG11527850.048.0986485
GGGTGTG11878600.045.9223337
GGTGTGT13358850.040.599318
GTGTGTT14495000.037.2593779
GTTGCGT60750.024.5821254
GTGTCGG48550.024.2117182
TTGGTTT7976950.022.4462533
TGTCGGG52400.021.5387653
TGGTTTT7146900.021.5188434
TTTGGTT9779400.020.8541342
TGTTGCG74400.020.2895743
GTTTGGT12548350.019.0103571
TGTGTTT14450800.018.63415110-11
TTGCGTG84050.018.2818555
GTGTTTG17330150.018.277641
Produced by FastQC (version 0.11.5)