FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122310701
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG19204750.076.455461
GTGTTGG19831750.073.5586242
TGTTGGG20156150.071.312393
GTTGGGT21248050.067.375644
TGGGTGT23106350.062.2161526
TTGGGTG23679250.061.434165
GGGTGTG23953200.059.79137
GGTGTGT25345800.056.2423638
GTGTGTT26958400.052.9023449
GTTGCGT116100.035.319924
TGTTGCG134700.030.177823
TTGCGTG149700.027.697795
TGTGTTT27536600.025.76070410-11
GTGTTTG32465050.023.3523110-11
TGTTTGG32712700.021.45767212-13
TGCGTGT219350.020.3274026
AAGACCG481350.020.18767594
GTTTGGT23192350.019.08337612-13
GTTTGGG15616150.018.48563812-13
GCGTGTG258050.018.2633887
Produced by FastQC (version 0.11.5)