FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64123429
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG18934800.068.969641
GTGTTGG20301200.064.027652
TGTTGGG21455850.059.1398123
GTTGGGT23167600.054.4383774
TTGGGTG26292500.048.397635
TGGGTGT26591950.047.853196
GGGTGTG30027150.042.3919877
GTTGCGT95100.037.1219024
GGTGTGT33897500.037.052918
GTGTGTT34524200.035.169049
TGTTGCG107900.032.8800663
GTGTCGG84950.032.6909942
GTCGGGT83800.032.0998884
TTGCGTG117550.030.486375
TGTCGGG91800.029.5864183
TGTGTCG103250.027.4027081
TCGGGTG108200.025.620955
GTGTGTC701350.025.6018229
TGCGTGT177900.021.0362226
CGGGTGT135300.019.9204646
Produced by FastQC (version 0.11.5)