FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65092337
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG23760900.069.056411
GTGTTGG24711200.066.113762
TGTTGGG25096600.064.02233
GTTGGGT26216100.060.9965174
TGGGTGT29019700.055.3411066
TTGGGTG29104550.055.3396645
GGGTGTG30595750.052.428477
GGTGTGT32918950.048.5702368
GTGTGTT33640500.047.397269
GTTGCGT119250.033.6209344
TGTTGCG120350.033.0774463
TTGCGTG128600.031.4723975
GTGTTTG32905800.025.7598410-11
TGTGTTT30967500.025.43632710-11
GTTTGGT20754500.024.37647212-13
TGTTTGG32804800.023.86435112-13
TTGGTTT11060000.022.37796214-15
GTGTCGG79550.022.1851582
GTTTGGG14709350.021.9044112-13
TGTCGGG77750.021.5679263
Produced by FastQC (version 0.11.5)