FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82894042
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG29516200.060.8568531
GTGTTGG30448650.058.88372
TGTTGGG30867800.057.2732353
GTTGGGT31954000.055.08614
TGGGTGT34648100.050.846196
TTGGGTG34882000.050.7295075
GGGTGTG35567400.049.388487
GGTGTGT38490050.045.5185058
GTGTGTT39729600.043.667849
GTTGCGT80650.026.0880344
GTGTTTG36941500.024.72156310-11
TGTGTTT34874200.024.54927810-11
GTTTGGT23911850.023.24934212-13
TGTTTGG37036500.022.99702512-13
TGTTGCG93500.022.840673
TTGCGTG99100.021.8470785
TTGGTTT13420550.021.2054214-15
GTTTGGG16499000.020.64786512-13
CGACATG198200.020.5318194
TTTGGTT17547250.020.28654514-15
Produced by FastQC (version 0.11.5)