FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74849496
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG26180300.062.142141
GTGTTGG27031700.060.1216662
TGTTGGG27469900.058.376463
GTTGGGT28452300.056.1284944
TGGGTGT30702400.052.089626
TTGGGTG30888700.051.9325145
GGGTGTG31375350.050.8115737
GGTGTGT33910450.046.695918
GTGTGTT34940900.044.8800549
GTGTTTG32135750.025.69933710-11
TGTGTTT30511150.025.39831510-11
GTTTGGT20763000.024.09729412-13
TGTTTGG32273050.023.90431212-13
TTGGTTT11936250.021.89752814-15
GTTTGGG14391250.021.55984512-13
TTTGGTT15432700.020.97218314-15
CGACATG149900.020.24322994
GAACGAC278350.020.23861794
TTGGGGG4715050.019.38881714-15
TGGTTTT10148700.019.38041516-17
Produced by FastQC (version 0.11.5)