FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44042480
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG8783250.078.5787351
GTGTTGG8903950.074.610262
TGTTGGG9106800.071.608033
GTTGCGT187300.070.148354
TGTTGCG192200.067.927093
GTTGGGT9682700.067.179894
TTGCGTG206650.063.931245
TGGGTGT10642250.061.4700436
TTGGGTG10751150.061.06135
GGGTGTG11247900.058.22297
GGTGTGT12058600.053.8944638
GCGTGTG263900.053.0257647
TGCGTGT262450.052.39836
GTGTGTT12718650.051.631449
CGTGTGT265300.051.1702888
GTGTTGC372250.036.0605552
TGTGTTT12621100.025.69839310-11
GTGTTTG14064100.024.90102210-11
TGTCGGG43300.024.799913
GTGTCGG46200.024.4256532
Produced by FastQC (version 0.11.5)